Barndioota is a locality and former town in the southern Flinders Ranges west of Hawker. The bounded locality of Barndioota corresponds to the cadastral Hundred of Barndioota and also includes the former town of Hookina. The town was surveyed in 1883 and declared ceased to exist in 1929.
The traditional owners of the area are the Adnyamathanha peoples.
In April 2016, Barndioota was proposed as a possible site for a government-owned nuclear waste storage facility. The proposal met opposition from locals, Aboriginal people and environmental groups. A decision on this is not expected soon.