Johnsonville is a town in the East Gippsland region of Victoria, Australia. It is located on the Princes Highway 18.9 km east from the city of Bairnsdale and 18.3 km west from the tourist resort and fishing port of Lakes Entrance. The town encompasses an area of approximately 5,617 hectares, 56 square kilometres or 13,879 acres.
Johnsonville forms one of the 224 cities, towns, villages and localities included within the scope of the East Gippsland Shire directive. The local member for the East Gippsland Region is Tim Bull. At the 2006 census, Johnsonville had a population of 586.