Lyonville is a town located in the Shire of Hepburn, Victoria, Australia. East of Daylesford on the Trentham road, the town takes its name from James Lyon who had arrived in the Glenlyon district in the 1860s. At the 2006 census, Lyonville had a population of 156.
Lyonville Post Office opened on 15 May 1882 and closed in 1993.
The Lyonville railway station was a stage of the Carlsruhe to Daylesford line until it closed in 1978. The Daylesford Spa Country Railway tourist railway is planning extensions of the track to Lyonville in the future.
The local attractions include Lyonville Hatha Yoga studio, Pero's Organic Wines and The Radio Springs Hotel.