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Holtze is an outer rural area of Darwin, Northern Territory, Australia. It is 22 km southeast of the Darwin CBD. Its local government area is the Municipality of Litchfield. The suburb is mostly a rural area, just north Palmerston. It may have been named for Maurice William Holtze (1840–1923), the botanist who established Darwin's Botanical Gardens, or his son Nicholas, who succeeded him as curator.
In September 2010, the Northern Territory Government announced that new Darwin’s prison precinct, Doug Owston Correctional Centre, will be built in Holtze about four kilometres north of Howard Springs Road. In July 2012, a road in Holtze was registered and named after prison officer, Reginald Anthony Willard (1943–1997), who worked at the correctional centre.