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Woolsthorpe is a small town in the Shire of Moyne, Victoria, Australia. It is situated at the intersection of the Woolsthorpe-Heywood, Koroit-Woolsthorpe, and Warrnambool-Caramut Roads, on the banks of Spring Creek. There is a local pub, the National Hotel. At the 2006 census, Woolsthorpe had a population of 694.
The nearest large town is Warrnambool 26.5 kilometres further south.
Woolsthorpe Post Office opened on 6 August 1861.
The local agriculture is primarily based on sheep and cattle.